Part:BBa_K1947022:Design

Improve the protein purification.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1192
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1112
  

Design Notes

GS linker is used to construct the fusion protein. Amilcp does not have a termination  codon.

Source

Protein splicing elements (termed inteins) were described as in-frame insertions in the Saccharomyces cerevisiae VMA1 gene.